Information
A Flongle Flow Cell (R10.4.1) is a consumable used with the Flongle adapter and MinION and GridION devices. It contains the proprietary sensor array and R10.4.1 nanopores suitable for all V14 kits using our Kit 14 chemistry.
As part of the Flongle expansion programme, for every 9 Flongle Flow Cells ordered, users will receive 3 additional flow cells on top of their current order to enable them to complete their experiments with confidence.
Please note: This offer is not available via our distributors and dealer stores.
This release supports a selection of our V14 kits recently released, including the Ligation Sequencing Kit V14 and Rapid Sequencing Kit V14. A full compatibilities list is available below.
Please note: you will require a Flongle adapter to utilise this product. Please ensure you have a Flongle adapter before purchasing the Flongle Flow Cells.
How it works:
A user simply adds their prepared library to the flow cell and starts their sequencing experiment.
Data is accumulated over time as DNA is sequenced by the nanopores. Individual read files are generated and are immediately available for analysis.
As data is streamed in real-time, a user can choose to stop a run as soon as sufficient data is gathered. A flow cell contains sufficient buffer to run for ~24 hours (under optimal conditions).
Shipping and logistics:
Flongle Flow Cells are packed individually; however, bulk order options are available. All orders can be split for delivery to best suit your needs.
Flow cells are sensitive to temperature. To ensure optimal results during use, consumables are shipped with cool packs. It is not unusual for the cool packs to be warm when you receive them. For optimal results, immediately store your new flow cells at their correct temperatures.
We recommend that flow cells should be stored between 2°C and 8°C until use. Flow cells should not be frozen at any point, or come into contact with frozen elements, such as the back panel of a refrigerator or frozen cool packs.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
Storage and stability:
Flow cell should be stored unopened between 2°C and 8°C until ready to use.
We recommend you check your flow cell before preparing your DNA library as part of the warranty process.
Flow cell returns:
Flongle Flow Cells are disposable and do not need to be returned to Oxford Nanopore Technologies. Please dispose of them as biological waste.
Workflow
Flongle Flow Cells are provided in a sealed pouch.
It is recommended that they are stored unopened between 2°C and 8°C until ready to use.
Prior to use, the flow cell can be checked by performing a flow cell check using MinKNOW (the MinION and GridION device software). The flow cell check will report the number of nanopores available for sequencing.
Once the flow cell check has been performed satisfactorily, the flow cell is ready to be used for experiments. The exact protocol and reagent kits used will depend on the experiment being run.
Safety and legal
Safety Data Sheet for Flongle Flow Cell (FLO-FLG114)
The warranty period for Flongle Flow Cells is four weeks from receipt by the customer.
Customers are required to perform a flow cell check prior to starting their sequencing experiment. Oxford Nanopore Technologies will replace any unused flow cell with fewer than 50 nanopores, when the result is reported within two days of performing the flow cell check, and when the storage recommendations have been followed.
There is a 9-month contract length available for all consumable purchases. For more information, please see the Nanopore Product Terms and Conditions.
What's in the box
Flongle Flow Cells are shipped in a sealed pouch.
Compatibility
Flongle R10.4.1 flow cells have been tested with the following sequencing kits:
- Ligation Sequencing Kit V14 (SQK-LSK114)
- Ligation Sequencing Kit XL V14 (SQK-LSK114-XL)
- Rapid Sequencing Kit V14 (SQK-RAD114)
- Rapid Barcoding Kit 24 V14 (SQK-RBK114.24)
- Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
- Rapid PCR Barcoding Kit 24 V14 (SQK-RPB114.24)
- Native Barcoding Kit 24 V14 (SQK-NBD114.24)
- Native Barcoding Kit 96 V14 (SQK-NBD114.96)
- 16S Barcoding Kit 24 V14 (SQK-16S114.24)
