Information
A PromethION Flow Cell (R10.4.1) is a consumable used with PromethION devices. It contains the proprietary sensor array, Application-Specific Integrated Circuit (ASIC), and R10 nanopores. The R10 series of nanopores contains a double reader-head, and is suitable for experiments where high consensus accuracy is required. Paired with the Kit 14 chemistry, R10.4.1 generates data at a modal accuracy above 99%. As a new product, it will be rapidly iterating.
This release supports all our V14 kits recently released, including the Ligation Sequencing Kit V14, Rapid Sequencing Kit V14 and barcoding. A full compatibilities list is available below.
Note: All flow cells will be shipped with flow cell light shields.
How it works:
A user simply adds their prepared library to the flow cell, installs the light shield and starts their sequencing experiment.
Data is accumulated over time as DNA or RNA is sequenced by the nanopores. Individual read files are generated and are immediately available for analysis.
As data is streamed in real-time, a user can choose to stop a run as soon as sufficient data is gathered. A flow cell contains sufficient buffer to run for ~72 hours (under optimal conditions) and a user can choose to run continuously or run, stop, wash, and load a new sample until the buffer and nanopores are exhausted.
Shipping and logistics:
PromethION Flow Cells are packaged in packs of 4. The flow cells are shipped in multiple set sizes, depending on the size of your project. All orders can be split for delivery to best suit your needs.
Flow cells and kits are shipped together at 2–8°C. The packaging is designed to protect the flow cells from freezing. Flow cells and sequencing kits are stable at room temperature for up to seven days, so users should not be concerned about their products should they arrive at ambient temperature.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
Storage and stability:
Please store your flow cells as follows:
- Room temperature: during Early Access we ask users to store their flow cells at 2-8°C. Flow cells are stable for a few days at room temperature, however we will formalise guidance during the course of product roll-out.
- 2-8°C: flow cells can be stored, unopened, at 2-8°C for 12 weeks.
We recommend you check your flow cell before preparing your DNA/RNA library as part of the warranty process.
Flow cell returns:
Four PromethION flow cells are supplied per box with a Returns insert which contains the seals and packaging for returning the flow cells to Oxford Nanopore where the materials are recycled. The box and returns packaging should be retained for returning the flow cell to Oxford Nanopore within 12 weeks of receipt.
More information on how to carry out a return can be found on the Nanopore Community.
Workflow
Prior to use, a Flow Cell Check should be run on the flow cell. This checks the environment for the flow cell chemistry has been maintained during shipping.
PromethION Flow Cells are provided in a sealed pouch and are shipped at a constant temperature. For long-term storage, it is recommended that they are kept in an environment not prone to large temperature fluctuations. The flow cells should be stored flat.
Once the Flow Cell Check has been performed satisfactorily, the flow cell is ready to be used for experiments. The exact protocol and reagent kits used will depend on the experiment being run.
Please return your flow cells and light shields to Oxford Nanopore Technologies after use.
What's in the box
PromethION flow cells are shipped in a sealed pouch in packs of four with light shields.
