Information

The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 96 unique barcodes and enough of the required adapters to make 10 libraries per barcode. The barcodes have been carefully designed and extensively purified to minimise the opportunity for cross-talk.

There are currently two protocols for this kit which are provided as examples of how the PCR Barcoding Expansion 1-96 could be applied for genomic and amplicon libraries. Please note that if you are barcoding cDNA or amplicons, these have to be pre-made in advance. These protocols are open for adaptation and optimisation for specific experimental requirements.

Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd Party Materials" tab for more detail.

The PCR Barcoding Expansion 1-96 features

Shipping and logistics

Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.

Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.

Workflow

Protocols

• Ligation sequencing V14 - PCR Barcoding (SQK-LSK114 with EXP-PBC001 or EXP-PBC096)
• Ligation sequencing DNA V14 - dual barcoding (SQK-NBD114.24 with EXP-PBC096)
• Ligation sequencing gDNA - PCR barcoding (SQK-LSK109 with EXP-PBC096)
• Ligation sequencing amplicons - PCR barcoding (SQK-LSK109 with EXP-PBC096)
• Ligation sequencing gDNA - PCR barcoding (SQK-LSK110 with EXP-PBC096)
• Ligation sequencing amplicons - PCR barcoding (SQK-LSK110 with EXP-PBC096)

To access the protocols, you will need to register for a Nanopore Community account.

Safety and legal

Safety Data Sheet - EXP-PBC096

What's in the box

There are 96 unique barcodes in the kit. Each kit provides material sufficient for 10 reactions of each barcode.

3rd party materials

In addition to the equipment and consumables used with the Ligation Sequencing Kit or Native Barcoding Kit 24 V14:

• LongAmp Hot Start Taq 2X Master Mix (NEB, M0533)
• Magnet for 96-well plates (for 96 barcodes)
• Multi-channel pipette (for 96 barcodes)
• 0.2 ml 96-well PCR plates and seals (for 96 barcodes)

Compatibility

The PCR Barcoding Expansion 1-96 can be used together with:

Kits

• Ligation Sequencing Kit V14 (SQK-LSK114)
• Ligation Sequencing Kit XL V14 (SQK-LSK114-XL)
• Native Barcoding Kit 24 V14 (SQK-NBD114.24)
• Ligation Sequencing Kit (SQK-LSK110)
• Ligation Sequencing Kit (SQK-LSK109)
• Flow Cell Wash Kit (EXP-WSH004)
• LFB Expansion (EXP-LFB001)
• SFB Expansion (EXP-SFB001)

Flow cells

• FLO-MIN106D
• FLO-MIN114
• FLO-PRO002
• FLO-PRO114M

Devices

• MinION Mk1B
• MinION Mk1C
• GridION
• PromethION