Information
The PCR Barcoding Expansion 1-12 is designed to allow the pooling and running together of multiple sequencing libraries on Oxford Nanopore sequencing devices. The kit includes adapters and Oxford Nanopore forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 12 unique barcodes and enough of the required adapters to make 6 libraries per barcode. The barcodes have been carefully designed and extensively purified to minimise the opportunity for cross-talk.
There is a protocol for this kit which is provided as an example of how the PCR Barcoding Expansion 1-12 could be applied for genomic and amplicon libraries. Please note that if you are barcoding gDNA or amplicons, these have to be pre-made in advance. These protocols are open for adaptation and optimisation for specific experimental requirements.
Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd party materials" tab for more detail.
The PCR Barcoding Expansion 1-12 features
| Feature | Property |
|---|
| Associated protocols | Please refer to the Protocols tab. |
| Pack size | 6 |
| Storage | −20°C to −30°C |
| Stability | Upon receipt, immediately store kits between −20°C and −30°C until you are ready to use them.
Kits are stable for up to 10 freeze/thaw cycles.
Oxford Nanopore Technologies deems the useful life of the product to be 3 months from receipt by the customer. |
Shipping and logistics
Expansion kits are sensitive to temperature. To maintain their temperature and ensure optimal results during use, consumables are shipped with cool packs. It is not unusual for the cool packs to be warm when you receive them. For optimal results, you should immediately store your new kits at their correct temperatures.
We recommend that expansion kits should be stored between −20°C and −30°C until you are ready to use them. Kits are stable for up to 10 freeze/thaw cycles.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
3rd party materials
In addition to the equipment and consumables used with the Ligation Sequencing Kit:
- LongAmp Hot Start Taq 2X Master Mix (NEB, M0533)
Compatibility
The PCR Barcoding Expansion 1-12 can be used together with:
Kits
- Ligation Sequencing Kit V14 (SQK-LSK114)
- Ligation Sequencing Kit XL V14 (SQK-LSK114-XL)
- Flow Cell Wash Kit (EXP-WSH004)
- LFB Expansion (EXP-LFB001)
- SFB Expansion (EXP-SFB001)
Flow cells
Devices
- MinION
- GridION
- PromethION
