Information
This kit is recommended for users who:
- Wish to multiplex up to 96 samples to reduce price per sample
- Want to achieve modal raw read accuracy of Q20+ (99%) and above
- Want to obtain duplex data
- Want to optimise their sequencing experiment for accuracy and output
- Need a PCR-free method of multiplexing to preserve additional information such as base modifications
- Require control over read length
- Would like to utilise upstream processes such as size selection or whole genome amplification
Please note that to use this kit, you will need to purchase additional 3rd party reagents: see the "3rd Party Materials" tab for more detail.
Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow cell.
Multiplexing samples onto one flow cell can reduce the cost per sample for a user. Below are some worked examples. Please note, this is an example and pricing depends on the kit purchased.
| No barcodes | 24 barcodes | 48 barcodes | 96 barcodes |
---|
Flow cell price | $500 | $500 | $500 | $500 |
Library price | $99 | $99 | $99 | $99 |
Barcodes price | - | $75 | $150 | $300 |
Price per sample | $599 | $28 | $15 | $9.36 |
The Native Barcoding Kit 96 V14 features: