Information
The LFB expansion contains additional Long Fragment Buffer (LFB) for use with sequencing experiments where shorter fragments (<3 kb) are to be removed.
The LFB Expansion properties:
| Feature | Property |
|---|
| Associated kits | • Ligation Sequencing Kit V14 (SQK-LSK114)
• Ligation Sequencing Kit XL V14 (SQK-LSK114-XL)
• PCR Barcoding Expansion 1-12 (EXP-PBC001)
• PCR Barcoding Expansion 1-96 (EXP-PBC096)
• Native Barcoding Auxiliary Kit V14 (EXP-NBA114)
• Native Barcoding Kit 24 V14 (SQK-NBD114.24)
• Native Barcoding Kit 96 V14 (SQK-NBD114.96) |
| Pack size | 4 reactions |
| Storage | −20°C to −30°C |
| Stability | Upon receipt, immediately store kits between −20°C and −30°C until you are ready to use them.
Kits are stable for up to 10 freeze/thaw cycles.
Oxford Nanopore Technologies deems the useful life of the product to be 3 months from receipt by the customer. |
Shipping and logistics
Expansion kits are sensitive to temperature. To maintain their temperature and ensure optimal results during use, consumables are shipped with cool packs. It is not unusual for the cool packs to be warm when you receive them. For optimal results, you should immediately store your new kits at their correct temperatures.
We recommend that expansion kits should be stored between −20°C and −30°C until you are ready to use them. Kits are stable for up to 10 freeze/thaw cycles.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
What's in the box
The LFB Expansion contains enough reagents for four reactions.
| Name | Acronym | No. of vials | Cap colour | Fill volume per vial |
|---|
| Long Fragment Buffer | LFB | 1 | Clear | 7.5 ml |
Compatibility
The LFB Expansion can be used together with:
Kits
- Ligation Sequencing Kit V14 (SQK-LSK114)
- Ligation Sequencing Kit XL V14 (SQK-LSK114-XL)
- PCR Barcoding Expansion 1-12 (EXP-PBC001)
- PCR Barcoding Expansion 1-96 (EXP-PBC096)
- Native Barcoding Auxiliary Kit V14 (EXP-NBA114)
- Native Barcoding Kit 24 V14 (SQK-NBD114.24)
- Native Barcoding Kit 96 V14 (SQK-NBD114.96)
Flow cells
Devices
- MinION
- GridION
- PromethION
