Information
There are three buffers that come into direct contact with a flow cell at the point of loading (Sequencing Buffer, Flow Cell Flush, and Library Beads or Library Solution). We have found that there is a very low level of contaminants seeping out of the plastic vials that contain these buffers, and this impacts the robustness of the Flongle flow cell system (MinION and PromethION are not impacted by this).
To rapidly deploy this to Flongle users, we have produced a Flongle Sequencing Expansion (EXP-FSE002), which can perform 12 Flongle flow cell loads in total.
The buffers in the Flongle Sequencing Expansion are shipped in glass vials to avoid the contaminant seeping issue. It is important to use these buffers when using Flongle Flow Cells.
Always refer to the latest protocols on the Nanopore Community for correct guidance on how to use our products.
Note: The Flongle Sequencing Expansion (EXP-FSE002) is only compatible with the Flongle Flow Cell (R10.4.1)
The Flongle Sequencing Expansion features
| Feature | Property |
|---|
| Associated kits | All V14 kits usable with Flongle Flow Cells (R10.4.1)† |
| Pack size | 12 reactions |
| Stability | Shipped at 2–8°C
Long-term storage -20 – -30°C
The warranty for this Early Access product is 3 months from receipt by the customer |
† This expansion is only compatible with Flongle Flow Cells (R10.4.1) and kits that can be used with this flow cell type.
Shipping and logistics
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
What's in the box
The Flongle Sequencing Expansion (EXP-FSE002) contains enough reagents for 12 reactions.
The buffers in the Flongle Sequencing Expansion are shipped in glass vials.
| Name | Acronym | Cap colour | No. of vials | Fill volume per vial (μl) |
|---|
| Sequencing Buffer | SB | Blue cap, white label | 1 | 250 |
| Library Beads | LIB | Blue cap, white label | 1 | 200 |
| Library Solution | LIS | Blue cap, white label | 1 | 200 |
| Flow Cell Flush | FB | Blue cap, white label | 1 | 1,600 |
Compatibility
The Flongle Sequencing Expansion (EXP-FSE002) can be used together with:
Flow cells
R10.4.1
Devices
- MinION Mk1B
- MinION Mk1C
- GridION